V Semester Examination May 2019
BACHELOR OF PHARMACY
Pharmaceutical Biotechnology
(BP-505T)
Time :- 3 hrs. Marks :- 80
Answer any FIVE questions :-
1. a) What is the PCR? Explain in detail with diagrams the steps involved in PCR. (10)
b) Explain the application of rDNA technology. (6)
2. a) Explain the Hybridoma technology with the well labelled diagram. (10)
b) What is the structure of Immunoglobulins. (6)
3. Write the short notes on any four of the following :- (4 × 4 = 16)
a) Sterilization methods of fermentation.
b) Production of Penicillin.
c) Mutation.
d) Bacterial vaccines.
e) Media components.
4. a) Describe the production of any two enzymes in detail. (8)
b) State the methods involved in the enzyme immobilization. (8)
5. a) Explain the application of Microbial Biotransformation. (8)
b) Define the Plasmids. What is their role in genetic engineering. (8)
6. Write down the short notes on any four of the following :- (4 × 4 = 16)
a) Cloning vectors
b) Application of Genetic Engineering
c) Difference between cellular and humoral immunity
d) Restriction Endonucleases
e) Insulin production
7. a) Define the Biosensors. Describe its various components in detail. (10)
b) Define the ELISA. Discuss its various types with the well labelled diagram. (6)
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